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smai linearized pegfp c2 vector  (TaKaRa)


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    Structured Review

    TaKaRa smai linearized pegfp c2 vector
    Smai Linearized Pegfp C2 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c2+vector/pAcGFP1-C2+Vector/pmc12626424-361-34-36
    Average 95 stars, based on 26 article reviews
    smai linearized pegfp c2 vector - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Pathogenic missense variation in PABPC1L/EPAB causes female infertility due to oocyte maturation arrest at the germinal vesicle stage.
    Article Snippet: Women undergoing controlled ovarian hyperstimulation prior to in vitro fertilization (IVF) are treated using various protocols to induce multiple follicular growths.. Complete failure of all oocytes to mature during IVF cycles is rare; however, it is a known cause of primary female infertility.. Recently, pathogenic variations in a few genes have been identified in women with oocyte maturation defects; however, the underlying genetic causes remain largely unknown.

    Article Title: ANGPTL3 regulates the peroxisomal translocation of SmarcAL1 in response to cell growth states
    Article Snippet: .. The GFP-Angptl3 construct was generated by cloning the cDNA of human ANGPTL3 into the pEGFP-C2 vector (Clontech) at EcoRI and ApaI sites. ..

    Article Title: ANGPTL3 regulates the peroxisomal translocation of SmarcAL1 in response to cell growth states
    Article Snippet: .. The GFP-Angptl3 construct was generated by cloning the cDNA of human ANGPTL3 into the pEGFP-C2 vector (Clontech) at EcoRI and ApaI sites. ..

    Article Title: ISWI chromatin remodeling complexes recruit NSD2 and H3K36me2 in pericentromeric heterochromatin.
    Article Snippet: .. The full-length and domain of interest were amplified by PCR using emGFPBAZ1B as a template and inserted into pEGFP-C2 vector (Takara Bio) digested with EcoRI using the In-Fusion HD Cloning Kit. .. One truncated vector without the PHD finger and BrD (emGFPΔPHD, BrD) was produced by inverse PCR using emGFP-BAZ1B as a template.

    Article Title: ANGPTL3 regulates the peroxisomal translocation of SmarcAL1 in response to cell growth states.
    Article Snippet: .. The GFP-Angptl3 construct was generated by cloning the cDNA of human ANGPTL3 into the pEGFP-C2 vector (Clontech) at EcoRI and ApaI sites. ..

    Article Title: VAV2 orchestrates the interplay between regenerative proliferation and ribogenesis in both keratinocytes and oral squamous cell carcinoma
    Article Snippet: .. For its construction, the cDNA encoding VAV2 Onc (Δ1 − 186) was liberated from plasmid pKES19 by digestion with BstXI, filled in, and cloned into the SmaI-linearized pEGFP-C2 vector (Clontech, cat. #632481). .. Luciferase activity was tested using a plasmid encoding luciferase under the regulation of the rRNA promoter (provided by L.-L. Chen, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 200031 Shanghai, China) and pRL-SV40 ( Renilla luciferase, obtained from Promega, cat. #E2231).

    Article Title: Identification of autophagy receptors for the Crohn’s disease-associated adherent-invasive Escherichia coli
    Article Snippet: .. NDP52 cDNA was cloned from T84 cells into the pEGFP-C2 vector (Clontech) using the SuperFi II DNA polymerase (Invitrogen) and the following primers: .. All transfections were performed using Lipofectamine 3000 RNAimax (Invitrogen) according to the manufacturer’s instructions.

    Article Title: STING guides the STX17-SNAP29-VAMP8 complex assembly to control autophagy
    Article Snippet: For GST-STX17 plasmid, STX17 was amplified and inserted into the pGEX-6p1 vector (GE Healthcare) by EcoRI and SalI. .. For GFP-STX17, GFP-SNAP29 and GFP-VAMP8 plasmids, STX17, SNAP29 and VAMP8 were amplified and inserted into the pEGFP-C2 vector (Clontech) by EcoRI and SalI. .. STING-CTD was amplified and inserted into the pET-22b (+) vector (Novagen) by NdeI and XhoI to generate STING-CTD-His; and inserted into the pGEX-6p1 vector (GE Healthcare) by EcoRI and SalI to generate GST-STING-CTD.

    Construct:

    Article Title: ANGPTL3 regulates the peroxisomal translocation of SmarcAL1 in response to cell growth states
    Article Snippet: .. The GFP-Angptl3 construct was generated by cloning the cDNA of human ANGPTL3 into the pEGFP-C2 vector (Clontech) at EcoRI and ApaI sites. ..

    Article Title: ANGPTL3 regulates the peroxisomal translocation of SmarcAL1 in response to cell growth states
    Article Snippet: .. The GFP-Angptl3 construct was generated by cloning the cDNA of human ANGPTL3 into the pEGFP-C2 vector (Clontech) at EcoRI and ApaI sites. ..

    Article Title: ANGPTL3 regulates the peroxisomal translocation of SmarcAL1 in response to cell growth states.
    Article Snippet: .. The GFP-Angptl3 construct was generated by cloning the cDNA of human ANGPTL3 into the pEGFP-C2 vector (Clontech) at EcoRI and ApaI sites. ..

    Generated:

    Article Title: ANGPTL3 regulates the peroxisomal translocation of SmarcAL1 in response to cell growth states
    Article Snippet: .. The GFP-Angptl3 construct was generated by cloning the cDNA of human ANGPTL3 into the pEGFP-C2 vector (Clontech) at EcoRI and ApaI sites. ..

    Article Title: ANGPTL3 regulates the peroxisomal translocation of SmarcAL1 in response to cell growth states
    Article Snippet: .. The GFP-Angptl3 construct was generated by cloning the cDNA of human ANGPTL3 into the pEGFP-C2 vector (Clontech) at EcoRI and ApaI sites. ..

    Article Title: ANGPTL3 regulates the peroxisomal translocation of SmarcAL1 in response to cell growth states.
    Article Snippet: .. The GFP-Angptl3 construct was generated by cloning the cDNA of human ANGPTL3 into the pEGFP-C2 vector (Clontech) at EcoRI and ApaI sites. ..

    Cloning:

    Article Title: ANGPTL3 regulates the peroxisomal translocation of SmarcAL1 in response to cell growth states
    Article Snippet: .. The GFP-Angptl3 construct was generated by cloning the cDNA of human ANGPTL3 into the pEGFP-C2 vector (Clontech) at EcoRI and ApaI sites. ..

    Article Title: ANGPTL3 regulates the peroxisomal translocation of SmarcAL1 in response to cell growth states
    Article Snippet: .. The GFP-Angptl3 construct was generated by cloning the cDNA of human ANGPTL3 into the pEGFP-C2 vector (Clontech) at EcoRI and ApaI sites. ..

    Article Title: ISWI chromatin remodeling complexes recruit NSD2 and H3K36me2 in pericentromeric heterochromatin.
    Article Snippet: .. The full-length and domain of interest were amplified by PCR using emGFPBAZ1B as a template and inserted into pEGFP-C2 vector (Takara Bio) digested with EcoRI using the In-Fusion HD Cloning Kit. .. One truncated vector without the PHD finger and BrD (emGFPΔPHD, BrD) was produced by inverse PCR using emGFP-BAZ1B as a template.

    Article Title: ANGPTL3 regulates the peroxisomal translocation of SmarcAL1 in response to cell growth states.
    Article Snippet: .. The GFP-Angptl3 construct was generated by cloning the cDNA of human ANGPTL3 into the pEGFP-C2 vector (Clontech) at EcoRI and ApaI sites. ..

    Amplification:

    Article Title: ISWI chromatin remodeling complexes recruit NSD2 and H3K36me2 in pericentromeric heterochromatin.
    Article Snippet: .. The full-length and domain of interest were amplified by PCR using emGFPBAZ1B as a template and inserted into pEGFP-C2 vector (Takara Bio) digested with EcoRI using the In-Fusion HD Cloning Kit. .. One truncated vector without the PHD finger and BrD (emGFPΔPHD, BrD) was produced by inverse PCR using emGFP-BAZ1B as a template.

    Article Title: STING guides the STX17-SNAP29-VAMP8 complex assembly to control autophagy
    Article Snippet: For GST-STX17 plasmid, STX17 was amplified and inserted into the pGEX-6p1 vector (GE Healthcare) by EcoRI and SalI. .. For GFP-STX17, GFP-SNAP29 and GFP-VAMP8 plasmids, STX17, SNAP29 and VAMP8 were amplified and inserted into the pEGFP-C2 vector (Clontech) by EcoRI and SalI. .. STING-CTD was amplified and inserted into the pET-22b (+) vector (Novagen) by NdeI and XhoI to generate STING-CTD-His; and inserted into the pGEX-6p1 vector (GE Healthcare) by EcoRI and SalI to generate GST-STING-CTD.

    Polymerase Chain Reaction:

    Article Title: ISWI chromatin remodeling complexes recruit NSD2 and H3K36me2 in pericentromeric heterochromatin.
    Article Snippet: .. The full-length and domain of interest were amplified by PCR using emGFPBAZ1B as a template and inserted into pEGFP-C2 vector (Takara Bio) digested with EcoRI using the In-Fusion HD Cloning Kit. .. One truncated vector without the PHD finger and BrD (emGFPΔPHD, BrD) was produced by inverse PCR using emGFP-BAZ1B as a template.

    Clone Assay:

    Article Title: VAV2 orchestrates the interplay between regenerative proliferation and ribogenesis in both keratinocytes and oral squamous cell carcinoma
    Article Snippet: .. For its construction, the cDNA encoding VAV2 Onc (Δ1 − 186) was liberated from plasmid pKES19 by digestion with BstXI, filled in, and cloned into the SmaI-linearized pEGFP-C2 vector (Clontech, cat. #632481). .. Luciferase activity was tested using a plasmid encoding luciferase under the regulation of the rRNA promoter (provided by L.-L. Chen, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 200031 Shanghai, China) and pRL-SV40 ( Renilla luciferase, obtained from Promega, cat. #E2231).

    Article Title: Identification of autophagy receptors for the Crohn’s disease-associated adherent-invasive Escherichia coli
    Article Snippet: .. NDP52 cDNA was cloned from T84 cells into the pEGFP-C2 vector (Clontech) using the SuperFi II DNA polymerase (Invitrogen) and the following primers: .. All transfections were performed using Lipofectamine 3000 RNAimax (Invitrogen) according to the manufacturer’s instructions.



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